Heilshorn Biomaterials Group

Materials Science & Engineering Department
Stanford University

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Implantable Materials for Regenerative Medicine


Injectable Materials for Cell Transplantation


In Vitro Mimics of
Tissue Architecture



Microfluidics for
Cell Migration and Chemotaxis


Biotemplates for
Inorganic Nanoparticles


Three-Dimensional Microenvironments
  Selected Publications
2014        2013        2012        2011        2010        2009        2008        2007        2006       Book Chapters
*links to articles are provided for convenience only; access to online material may require a subscription
2014
VanDersarl JJ., Mehraeen S., Schoen A., Heilshorn SC., Spakowitz A., Melosh NA. Rheology and Simulation of 2-Dimensional Clathrin Protein Network Assembly. Soft Matter, Accepted.

Chang DT., Chai R., DiMarco R., Heilshorn SC., Cheng A. Protein-engineered hydrogel encapsulation for 3D culture of murine cochlea. Otology & Neurotology, Accepted.

Mulyasasmita W., Cai L., Dewi R., Jha A., Ullmann SD., Luong RH., Huang NF., Heilshorn SC. Avidity-controlled hydrogels for injectable co-delivery of induced pluripotent stem cell-derived endothelial cells and growth factors. Journal of Controlled Release, Accepted 8 May 2014. DOI: 10.1016/jconrel.2014.05.015. link to article   link to pdf

Xu H., Ferreira MM., Heilshorn SC. Small-molecule axon-polarization studies enabled by a shear-free microfluidic gradient generator. Lab on a Chip, 2014, 14(12): 2047-2056. link to article   link to pdf

McLaughlin LM., Xu H., Carden SE., Fisher S., Reyes M., Heilshorn SC., Monack DM. A microfluidic-based genetic screen to identify microbial virulence factors that inhibit dendritic cell migration. Integrative Biology, 2014, (6): 438-449. link to article   link to pdf

Madl CM., Mehta M., Duda GN., Heilshorn SC., Mooney DJ. Presentation of BMP-2 Mimicking Peptides in 3D Hydrogels Directs Cell Fate Commitment in Osteoblasts and Mesenchymal Stem Cells. Biomacromolecules, Accepted 8 Jan 2014. DOI: 10.1021/bm401726u. link to article   link to pdf

Cai L., Dinh CB, Heilshorn SC. One-pot synthesis of elastin-like polypeptide hydrogels with grafted VEGF-mimetic peptides. Biomaterials Science, 2014, (2): 757-765. link to article   link to pdf

Cai L., Heilshorn SC. Designing ECM-mimetic materials using protein engineering. Acta Biomaterialia, 2014, 10(4): 1751-1760. link to article   link to pdf

DiMarco R, Su J, Yan K, Dewi R, Kuo CJ, Heilshorn SC. Engineering of Three-Dimensional Microenvironments to Promote Contractile Behavior in Primary Intestinal Organoids. Integrative Biology, 2014, 6(2):127-142. link to article   link to pdf
          *This article was featured on the cover of Integrative Biology
2013
Mulyasasmita W, Cai L, Hori Y, Heilshorn SC. Avidity-Controlled Delivery of Angiogenic Peptides from Injectable Molecular-Recognition Hydrogels. Tissue Engineering, Part A, Accepted 4 Dec 2013. link to article  link to pdf

Schoen AP, Huggins KNL, Heilshorn SC. Engineered clathrin nanoreactors provide tunable control over gold nanoparticle synthesis and clustering. Journal of Materials Chemistry B, 2013, 1:6662-6669. link to article  link to pdf

Huang NF, Dewi RE, Okogbaa J, Lee JC, Rufaihah A, Heilshorn SC, Cooke JP. Chemotaxis of human induced pluripotent stem cell-derived endothelial cells. American Journal of Translational Research, 2013, 5(5):510-520. link to article   link to pdf
Shamloo A, Manchandia M, Ferreira M, Mani M, Nguyen C, Jahn T, Weinberg K, Heilshorn SC. Complex chemoattractive and chemorepellent Kit signals revealed by direct imaging of murine mast cells in microfluidic gradient chambers. Integrative Biology, 2013, 5:1076-1085. link to article   link to pdf

Chung C, Pruitt BL, Heilshorn SC. Spontaneous cardiomyocyte differentiation of mouse embryoid bodies regulated by hydrogel crosslink density. Biomaterials Science, 2013, 1(10): 1082-1090. link to article   link to pdf
Schoen AP, Cordella N, Mehraeen S, Arunagirinathan MA, Spakowitz AJ, Heilshorn SC. Dynamic remodeling of disordered protein aggregates is an alternative pathway to achieve robust self-assembly of nanostructures. Soft Matter, 2013, 9(38): 9137-9145.  link to article   link to pdf
Schoen AP, Hommersom B, Heilshorn SC, Leunissen ME. Tuning colloidal association with specific peptide interactions. Soft Matter, 2013, 9(29): 6781-6785. link to article   link to pdf
Lampe KJ, Antaris AL, Heilshorn SC. Design of 3D engineered protein hydrogels for tailored control of neurite growth. Acta Biomaterialia, 2013, 9:5590-5599. link to article   link to pdf
Parisi-Amon A, Mulyasasmita W, Chung C, Heilshorn SC. Protein-engineered injectable hydrogel to improve retention of transplanted adipose-derived stem cells. Advanced Healthcare Materials, 2013, 2:428-432. link to article   link to pdf
Xu H, Heilshorn SC. Microfluidic investigation of BDNF enhanced neural stem cell chemotaxis in CXCL12 gradients. Small, 2013, 9:585-595. link to article   link to pdf
Benitez P, Sweet JA, Fink H, Chennazhi KP, Nair SK, Enejder A, Heilshorn SC. Sequence-specific crosslinking of electrospun elastin-like protein preserves bioactivity and native-like mechanics. Advanced Healthcare Materials, 2013, 2:114-118. link to article   link to pdf
2012
Chung C, Lampe K, Heilshorn SC. Tetrakis (hydroxyl methyl) phosphonium chloride as a covalent crosslinking agent for cell encapsulation within protein-based hydrogels. Biomacromolecules, 2012, 13:3912-3916. link to article   link to pdf
Chung C, Anderson E, Reijo Pera R, Pruitt BL, Heilshorn SC. Hydrogel crosslinking density regulated temporal contractility of human embryonic stem cell-derived cardiomyocytes in 3D cultures. Soft Matter, 2012, 8:10141-10148.  link to article   link to pdf
DiMarco RL, Heilshorn SC. Multifunctional materials through modular protein engineering. Advanced Materials, 2012, 24:3923-3940.  link to article   link to pdf
Sengupta D, Gilbert P, Johnson K, Blau HM, Heilshorn SC. Protein-engineered biomaterials to generate human skeletal muscle mimics. Advanced Healthcare Materials, 2012, 1:785-789.  link to article   link to pdf
Yan KS, Chia LA, Li X, Ootani A, Su J, Lee JY, Su N, Luo Y, Heilshorn SC, Amieva MR, Sangiorgi E, Capecchi MR, Kuo C. The intestinal stem cell markers Bmi1 and Lgr5 identify two functionally distinct populations. Proceedings of the National Academy of Sciences USA, 2012, 109:466-471.  link to article   link to pdf
Raphel J, Parisi-Amon A, Heilshorn SC. Photoreactive elastin-like proteins for use as versatile bioactive materials and surface coatings. Journal of Materials Chemistry, 2012, 22:19429-19437.  link to article   link to pdf
*This is a peer-reviewed, invited article for a special issue on Materials for Biosurfaces
Lampe KJ, Heilshorn SC. Building stem cell niches from the molecule up through engineered peptide materials. Accepted for publication, Neuroscience Letters, 2012, 519:138-146.  link to article   link to pdf
*This is a peer-reviewed, invited review article for a special issue on Bioengineered Approaches to Nervous System Repair
Aguado BA, Mulyasasmita W, Su J, Lampe KJ, Heilshorn SC. Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. Tissue Engineering Part A, 2012, 18:806-815. link to article   link to pdf
Shamloo A, Xu H, Heilshorn SC. Mechanisms of VEGF-induced path-finding by endothelial sprouts in biomaterials. Tissue Engineering Part A, 2012, 18:320-330link to article   link to pdf
2011
Schoen AP, Schoen DT, Huggins KN, Arunigirinathan MA, Heilshorn SC. Template Engineering Through Epitope Recognition: A modular, biomimetic strategy for inorganic nanomaterial synthesis. Journal of the American Chemical Society, 2011, 133:18202-18207link to article   link to pdf
Mulyasasmita W, Lee JS, Heilshorn SC. Molecular-level engineering of protein physical hydrogels for predictive sol-gel phase behavior. Biomacromolecules, 2011, 12:3406-3411. link to article   link to pdf
Nevill JT, Mo A, Cord BJ, Palmer TD, Poo MM, Lee LP, Heilshorn SC. Vacuum soft lithography for one-step patterning of multiple biomolecules to direct neuronal polarization. Soft Matter, 2011, 7:343-347. link to article   link to pdf
*This article was featured on the cover of Soft Matter
Romano N, Sengupta D, Chung C, Heilshorn S. Protein-engineered biomaterials: Nanoscale mimics of the extracellular matrix. Biochimica et Biophysica Acta, 2011, 1810:339-349. link to article   link to pdf
*This is a peer-reviewed, invited review article for a special issue on Emerging Applications of Nanotechnologies in Biomedicine
2010
Kuhnert F, Mancuso MR, Shamloo A, Wang HT, Choksi V, Florek M, Su H, Fruttiger M, Young WL, Heilshorn SC, Kuo CJ. Essential regulation of CNS angiogenesis by the orphan G protein-coupled receptor GPR124. Science, 2010, 330:985-989. link to article   link to pdf
Schoen DT*, Schoen AP*, Hu L, Kim HS, Heilshorn SC, Cui Y. High Speed Water Sterilization Using One Dimensional Nanostructures. Nano Letters, 2010, 10:3628-3632. link to article   link to pdf
Shamloo A, Heilshorn SC. Matrix Density Mediates Polarization and Lumen Formation of Endothelial Sprouts in VEGF Gradients. Lab on a Chip, 2010, 10:3061-3068. link to article   link to pdf
*This article was featured on the cover of Lab on a Chip
Shelly M, Lim BK, Cancedda L, Heilshorn SC, Gao H, Poo MM. Local and long-range reciprocal regulation of cAMP and cGMP in axon/dendrite formation. Science, 2010, 327:547-552. link to article   link to pdf
Sengupta D, Heilshorn SC. Protein-engineered biomaterials: Highly tunable tissue engineering scaffolds. Tissue Engineering Part B: Reviews, 2010, 16:285-293. link to article   link to pdf
*This article was featured on the cover of Tissue Engineering Part B: Reviews
Straley KS, Wong Po Foo C, Heilshorn SC. Biomaterial design strategies for the treatment of spinal cord injuries. Journal of Neurotrauma, 2010, 27:1-19. link to article   link to pdf
2009
Wong Po Foo C, Lee JS, Mulyasasmita W, Parisi-Amon A, Heilshorn SC. Two-component protein-engineered physical hydrogels for cell encapsulation. Proceedings of the National Academy of Sciences USA, 2009, 106:22067-22072. link to article   link to pdf
Straley KS and Heilshorn SC. Dynamic, three-dimensional pattern formation within enzyme-responsive hydrogels. Advanced Materials, 2009, 21:4148-4152.   Press release   link to article   link to pdf
*This article was featured on the cover of Advanced Materials
Straley KS and Heilshorn SC. Design and adsorption of modular engineered proteins to prepare customized, neuron-compatible coatings. Frontiers in Neuroengineering, 2009, 2:9. link to article   link to pdf
Wilson RJ, Hu W, Wong Po Foo C, Koh AL, Gaster RS, Earhart CM, Fu A, Heilshorn SC, Sinclair R, Wang SX. Formation and properties of magnetic chains for 100 nm nanoparticles used in separations of molecules and cells. Journal of Magnetism and Magnetic Materials, 2009, 321:1452-1458. link to article   link to pdf
Wang S, Wong Po Foo C, Warrier A, Poo MM, Heilshorn SC, Zhang X. Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture environments. Biomedical Microdevices, 2009, 11:1127-1134. link to article   link to pdf
Straley K, Heilshorn SC. Independent tuning of multiple biomaterial properties using protein engineering.  Soft Matter, 2009, 5:114-124. link to article   link to pdf
2008
Shamloo A, Ma N, Poo MM, Sohn L, Heilshorn SC. Endothelial cell polarization and chemotaxis in a microfluidic device.   Lab on a Chip, 2008, 8:1292-1299. link to article   link to pdf
2007
Shelly M, Cancedda L, Heilshorn S, Sumbre G, Poo M.  LKB1/STRAD promotes axon initiation during neuronal polarization.  Cell, 2007, 129:565-577. link to article   link to pdf
Carrico IS, Maskarinec SA, Heilshorn SC, Mock ML, Liu JC, Nowatzki PJ, Franck C, Ravichandran G, Tirrell D.  Lithographic patterning of photoreactive cell-adhesive proteins.   Journal of the American Chemical Society, 2007, 129:4874-4875. link to article   link to pdf
2006 and prior
Heilshorn SC,  Liu JC, DiZio KA, Tirrell DA.  Cell-binding domain context affects cell behavior on engineered proteins.  Biomacromolecules, 2005, 6:318-323. link to article   link to pdf
Liu JC, Heilshorn SC, Tirrell DA.  Comparative cell response to artificial extracellular matrix proteins containing the RGD and  CS5 cell-binding domains.  Biomacromolecules, 2004, 5:497-504. link to article   link to pdf
Heilshorn SC, DiZio KA, Welsh ER, Tirrell DA.  Endothelial cell adhesion to the fibronectin CS5 domain in artificial  extracellular matrix proteins.  Biomaterials, 2003, 24:4245-4252. link to article   link to pdf
Glenn RW, Sine MR, Evans MD, Carethers ME, Heilshorn SC "Liquid personal cleansing compositions which contain a  complex coacervate for improved sensory perception", U.S. patents #6,028,043 (2000), #5,932,528 (1999), #5,858,938 (1999),  #5,716,920 (1998). Assignee: The Procter & Gamble Company.
*links to articles are provided for convenience only; access to online material may require a subscription
Book Chapters
Benitez P, Heilshorn SC. (2013) "Microfluidic devices for quantifying the role of soluble gradients in early angiogenesis." Ed. Reinhart-King CA, Mechanical and Chemical Signaling in Angiogenesis, Heidelberg, Germany, Springer.
Raphel J, Parisi-Amon AP, Heilshorn SC. (2012) "Protein-Engineered Hydrogels." Eds. Taubert A, Mano J, Rodriquez-Cabello JC, Biomaterials Surface Science, Mannheim, Germany, Wiley-VCH Verlag.
Parisi-Amon A, Heilshorn SC. (2012) "Engineered Protein Biomaterials." Eds. Bronzino JD, Peterson DR, Section Eds. Fisher JP, Mikos AG, Biomedical Engineering Handbook, 4th Edition, Volume 4, Boca Raton, FL, CRC Press, Taylor & Francis Group.
Greenwood-Goodwin M, Heilshorn SC. (2012) "Hydrogels from Protein Engineering." Ed. Mano JF, Biomimetic Approaches for Biomaterials Development. Mannheim, Germany, Wiley-VCH Verlag.
Mulyasasmita W, Heilshorn SC. (2011) "Protein-Engineered Biomaterials: Synthesis and Characterization." Eds. Ducheyne P, Healy K, Hutmacher DW, Grainger DW, Kirkpatrick CJ, Comprehensive Biomaterials. Oxford, UK, Elsevier Science.
Wong Po Foo C, Heilshorn SC. (2010) "Protein Engineered Biomaterials." Eds. Park SJ, Cochran JR, Protein Engineering. Boca Raton, FL, CRC Press, Taylor & Francis Group.



Materials Science & Engineering DepartmentStanford University

Updated 6/12/2014